Concentration Assay:Article Title: The Interaction between Macrophages and Triple‐negative Breast Cancer Cells Induces ROS‐Mediated Interleukin 1α Expression to Enhance Tumorigenesis and Metastasis
Article Snippet: Antibody name Company Catalog # Application and concentration IL1, mouse Santa Cruz sc-9983 WB (1:500), IF (1:100), IHC (1:100) IL1, mouse Santa Cruz sc-32294 WB (1:500), IF (1:100) IL8, rabbit CST* 94407 WB (1:1000), IF (1:100) IL1R1, mouse Santa Cruz sc-393998 WB (1:1000) IRAK4, rabbit CST 4363S WB (1:1000) p-IRAK4, rabbit CST 11927S WB (1:1000) p-MKK4 (Ser257), rabbit CST 4514 WB (1:1000) JNK, rabbit CST 9252 WB (1:1000) p-JNK (Thr183/Tyr185), rabbit CST 9251 WB (1:1000) c-Jun, rabbit CST 9165 WB (1:1000) p-c-Jun (Ser73) CST 3270 WB (1:1000) ERK1/2, rabbit CST 4695 WB (1: 1000) p-ERK1/2 (Thr202/Tyr204), rabbit CST 4370 WB (1: 1000) ZEB1, rabbit Novus NBP1-88845 WB (1:1000) Vimentin, rabbit CST 5741 WB (1:1000) IB, mouse CST 4814 WB (1:1000) p-IB (Ser32/36), mouse CST 9246 WB (1:1000) NF-B, rabbit CST 8242 WB (1:1000) p-NF-B (Ser536), rabbit CST 3033 WB (1:1000) AKT, rabbit CST 4691 WB (1:1000) p-AKT (Ser473), rabbit CST 4060 WB (1:1000) p38, rabbit CST 8690 WB (1:1000) p-p38 (Thr180/Tyr182), rabbit CST 4511 WB (1:1000) GAPDH, rabbit CST 2118 WB (1:5000) F4/80, rat Abcam ab6640 IF (1:100) CD204, rabbit Thermo PA5-22596 IF (1:100), IHC (1:500) CD206, goat R&D AF2535 IF (1:100) CD163, rabbit Abcam Ab18442 IF (1:100), IHC (1:500) Goat anti-Rabbit IgG (H+L) 2nd Ab, HRP Bio-Rad 1706515 WB (1:5000) Goat anti-Mouse IgG (H+L)- 2nd Ab, HRP Invitrogen 31430 WB (1:5000) Goat anti-Rat IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A11006 IF (1:100) Donkey anti-Goat IgG (H+L) 2nd Ab, Alexa Fluor Plus 594 Invitrogen A11058 IF (1:100) Goat anti-Rabbit IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A32731 IF (1:100) Goat anti-Mouse IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A11029 IF (1:100) *CST, Cell Signaling Technology, USA.
Western Blot:Article Title: The Interaction between Macrophages and Triple‐negative Breast Cancer Cells Induces ROS‐Mediated Interleukin 1α Expression to Enhance Tumorigenesis and Metastasis
Article Snippet: Antibody name Company Catalog # Application and concentration IL1, mouse Santa Cruz sc-9983 WB (1:500), IF (1:100), IHC (1:100) IL1, mouse Santa Cruz sc-32294 WB (1:500), IF (1:100) IL8, rabbit CST* 94407 WB (1:1000), IF (1:100) IL1R1, mouse Santa Cruz sc-393998 WB (1:1000) IRAK4, rabbit CST 4363S WB (1:1000) p-IRAK4, rabbit CST 11927S WB (1:1000) p-MKK4 (Ser257), rabbit CST 4514 WB (1:1000) JNK, rabbit CST 9252 WB (1:1000) p-JNK (Thr183/Tyr185), rabbit CST 9251 WB (1:1000) c-Jun, rabbit CST 9165 WB (1:1000) p-c-Jun (Ser73) CST 3270 WB (1:1000) ERK1/2, rabbit CST 4695 WB (1: 1000) p-ERK1/2 (Thr202/Tyr204), rabbit CST 4370 WB (1: 1000) ZEB1, rabbit Novus NBP1-88845 WB (1:1000) Vimentin, rabbit CST 5741 WB (1:1000) IB, mouse CST 4814 WB (1:1000) p-IB (Ser32/36), mouse CST 9246 WB (1:1000) NF-B, rabbit CST 8242 WB (1:1000) p-NF-B (Ser536), rabbit CST 3033 WB (1:1000) AKT, rabbit CST 4691 WB (1:1000) p-AKT (Ser473), rabbit CST 4060 WB (1:1000) p38, rabbit CST 8690 WB (1:1000) p-p38 (Thr180/Tyr182), rabbit CST 4511 WB (1:1000) GAPDH, rabbit CST 2118 WB (1:5000) F4/80, rat Abcam ab6640 IF (1:100) CD204, rabbit Thermo PA5-22596 IF (1:100), IHC (1:500) CD206, goat R&D AF2535 IF (1:100) CD163, rabbit Abcam Ab18442 IF (1:100), IHC (1:500) Goat anti-Rabbit IgG (H+L) 2nd Ab, HRP Bio-Rad 1706515 WB (1:5000) Goat anti-Mouse IgG (H+L)- 2nd Ab, HRP Invitrogen 31430 WB (1:5000) Goat anti-Rat IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A11006 IF (1:100) Donkey anti-Goat IgG (H+L) 2nd Ab, Alexa Fluor Plus 594 Invitrogen A11058 IF (1:100) Goat anti-Rabbit IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A32731 IF (1:100) Goat anti-Mouse IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A11029 IF (1:100) *CST, Cell Signaling Technology, USA.
Immunohistochemistry:Article Title: The Interaction between Macrophages and Triple‐negative Breast Cancer Cells Induces ROS‐Mediated Interleukin 1α Expression to Enhance Tumorigenesis and Metastasis
Article Snippet: Antibody name Company Catalog # Application and concentration IL1, mouse Santa Cruz sc-9983 WB (1:500), IF (1:100), IHC (1:100) IL1, mouse Santa Cruz sc-32294 WB (1:500), IF (1:100) IL8, rabbit CST* 94407 WB (1:1000), IF (1:100) IL1R1, mouse Santa Cruz sc-393998 WB (1:1000) IRAK4, rabbit CST 4363S WB (1:1000) p-IRAK4, rabbit CST 11927S WB (1:1000) p-MKK4 (Ser257), rabbit CST 4514 WB (1:1000) JNK, rabbit CST 9252 WB (1:1000) p-JNK (Thr183/Tyr185), rabbit CST 9251 WB (1:1000) c-Jun, rabbit CST 9165 WB (1:1000) p-c-Jun (Ser73) CST 3270 WB (1:1000) ERK1/2, rabbit CST 4695 WB (1: 1000) p-ERK1/2 (Thr202/Tyr204), rabbit CST 4370 WB (1: 1000) ZEB1, rabbit Novus NBP1-88845 WB (1:1000) Vimentin, rabbit CST 5741 WB (1:1000) IB, mouse CST 4814 WB (1:1000) p-IB (Ser32/36), mouse CST 9246 WB (1:1000) NF-B, rabbit CST 8242 WB (1:1000) p-NF-B (Ser536), rabbit CST 3033 WB (1:1000) AKT, rabbit CST 4691 WB (1:1000) p-AKT (Ser473), rabbit CST 4060 WB (1:1000) p38, rabbit CST 8690 WB (1:1000) p-p38 (Thr180/Tyr182), rabbit CST 4511 WB (1:1000) GAPDH, rabbit CST 2118 WB (1:5000) F4/80, rat Abcam ab6640 IF (1:100) CD204, rabbit Thermo PA5-22596 IF (1:100), IHC (1:500) CD206, goat R&D AF2535 IF (1:100) CD163, rabbit Abcam Ab18442 IF (1:100), IHC (1:500) Goat anti-Rabbit IgG (H+L) 2nd Ab, HRP Bio-Rad 1706515 WB (1:5000) Goat anti-Mouse IgG (H+L)- 2nd Ab, HRP Invitrogen 31430 WB (1:5000) Goat anti-Rat IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A11006 IF (1:100) Donkey anti-Goat IgG (H+L) 2nd Ab, Alexa Fluor Plus 594 Invitrogen A11058 IF (1:100) Goat anti-Rabbit IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A32731 IF (1:100) Goat anti-Mouse IgG (H+L) 2nd Ab, Alexa Fluor Plus 488 Invitrogen A11029 IF (1:100) *CST, Cell Signaling Technology, USA.
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